THERMAL TOLERANCE PHENOTYPE IN CDC13-1 EXO1 HETEROZYGOUS DIPLOIDS OF S. CEREVISIAE IS A DOMINANT TRAIT

Background: Telomeric DNA is found at the end of eukaryotic chromosomes, where they play a role in protecting chromosome and integrity genome organism through activity telomerase. Saccharomyces cerevisiae exists two genotypes: haploid diploid. Temperature sensitive point mutation on cdc13 gene each genotype deletion exo1 (cdc13-1Exo1 mutants) give rise to mutant survivors enhanced temperatures. The mode inheritance thermal tolerance allele heterozygous diploid not known. Materials Methods: We constructed diploids by mating temperature strains opposite type cdc13-1 exo1: LEU with resistant exo1::HIS. crosses were 1296x3182 (D) 2561x3181 (C). Using sterile stick, smear one strain was made YEPD plates labelled C2, C8, C10, D4, D10, D113. A another previous strain. They mixed allowed mate for six hours, before culturing media lacking Luecine Histidine (–L –H) purify confirm that are diploids. After confirmation, loop full aliquot streaked leucine histidine (-L, -H) cultured 370C check number viable colonies from (cfu). Result: D thrived there significant difference yield when compared C P-value 0.05. Conclusion: growth D10 as shown plate 3.1 establishes that, inherited exo1heterozygous dominant phenotype, its 370C.